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[71] [72] Sources [edit] Bacteria and archaea [edit] Vitamin B 12 is produced in nature by certain bacteria and archaea

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These within-class switches are generally simpler than cross-class transitions because the underlying mechanism of action remains the same

The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)