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After blocking, the membrane was incubated overnight at 4 C with the specified primary antibodies, including TRPM2 antibody at a dilution of 1:1000 and GAPDH antibody at a dilution of 1:3000

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Finally, in a meta-analysis of randomized trials on the use of vitamin B supplements for homocysteine reduction, folate supplementation had no significant effect on the 5-year incidence of CHD

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