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Baliou S, Adamaki M, Ioannou P, Pappa A, Panayiotidis MI, Spandidos DA

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

The Recommended Daily Allowance (RDA) of the National Academy of Science for niacin during lactation is 20 mg

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Follow the dispensing pharmacys refrigeration, storage, and disposal directions