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The samples are centrifuged (5,000 rpm, 5 min), 30 L of the resulting supernatant and 30 L of the Ellmans reagent are added to 1 mL of phosphate buffer (pH 7.8), and the optical density is measured at =412 nm and =700 nm [19]
Early evidence suggests GLP-1 medications may also reduce neuroinflammation, which has been linked to disrupted sleep regulation in individuals with metabolic disease (ref, ref)
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