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For clinical evaluation, the study used a visual analog scale (VAS 0 10 pain scale) (first step, rest, activity) and Foot Function Index (FFI) (pain, disability, activity)

Additionally, treatment with 10 nM E2, for 7 days, stimulated differentiation to the neuronal lineage, and inhibited differentiation to astrocytes (Zhang et al., 2019)

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To obtain the necessary number of animals for the experiment, eight Wistar females were mated with eight Wistar males (1:1, individual cages) after checking the estrous cycle adapted from Marcondes and collaborators (2002)