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While trichotillomania rates did not significantly differ between genders, the mean age of onset was 17.7 years, with a significant difference between males (19.0 years) and females (14.8 years)
Here is a summary of how topical GHK-Cu was administered in three select studies: In a 1998 study by Abdulghani et al., GHK-Cu was found to have a significant effect on collagen production when applied as a cream to the thigh area for one month
Antibody-stained protein signal was amplified and visualized using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher #34578) and imaged with a ChemiDoc MP Imaging system (Bio-Rad)
Leave it for 15-20 minutes before rinsing with water
Lewis et al., 2018)
