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After adding 120 L of 250 mM sucrose, the mitochondria were centrifuged at 10,000 g for 15 min

This gradual titration lets you assess individual tolerance while keeping injection volumes practical

474 Moreover, these agents reduce the risks of myocardial inflammation and fibrosis and improve cardiac structure and function

Injecting on the same day each week, at roughly the same time, is the most important timing variable

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