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The medium was then replaced with a Dulbeccos Modified Eagle Medium (DMEM) medium containing H 2 O 2 (250 M) to induce oxidative stress and cultured for another 24 h

The start date of prescription were identified using the Observational Medical Outcomes Partnership Common Data Model (CDM)

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Clinical profile of Angelman syndrome at different ages

These therapeutic differences may reflect distinct metabolic profiles and could act as confounders, as some antidiabetic agents might indirectly affect ocular surface homeostasis through hydration status or inflammatory pathways