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Carbonyls were derivatised with 2,4-dinitrophenyl hydrazine (Sigma-Aldrich) and coated on an ELISA plate (Nunc-Immuno plate maxisorp, Nunc, Roskilde, Denmark)
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The processes of passage through the bloodbrain barrier and penetration into deeper brain regions differ between the GLP-1R agonists (Salinas et al., 2018)
Following washing, cortices were mechanically dissociated with repeated mixing using a 5 mL serological pipette and the resulting cell-suspension diluted with Opti-MEM (Life Technologies) supplemented with glucose (20 mM) and Antimicrobial-Antifungal to achieve a final tissue concentration of 0.14 mouse cortical hemispheres
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