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Researchers should note that some immortalized cell lines (particularly HEK293) express relatively low IGF-1R and may require receptor overexpression constructs to achieve meaningful signaling responses

FDA staff briefing documents have already recommended against all seven, citing a lack of human safety data for most of them

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With exception of p -cresol sulfate (a known protein degradation product in bacterial metabolism 37 ) and 4-hydroxphenylpyruvate, the top accumulating metabolites belonged to mono- and polyunsaturated fatty acids (Fig