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Huang Y, Cai X, Mai W, Li M, Hu Y
Following centrifugation, 50 l of suspension of nuclei were placed on each slide mixed with 50 l 1% low melting point agarose at 55 C, covered with 20 50 mm cover glass and stated on ice for 5 min
four ONTs bring in US$ 1.24 bn for AlnylamONTs are widely applied to treat neurodegenerative diseases, such as Duchenne muscular dystrophy (DMD)
The application is super straightforward just peel and stick
For instance, single nucleus sequencing of the entorhinal cortex of the hippocampus from AD brains reveals a repression of ApoE expression in astrocytic subpopulations and also oligodendrocyte precursor cells, but an increase in microglial expression (Grubman et al., 2019)
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