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Test compounds were incubated in enriched 3-6 hpi ring-stage artemisinin resistant (Cam3.II R539T , Cam3.II C580Y and MRA1240) or sensitive (Cam3.II REV and MRA1239) parasites and the level of peroxide activation determined by measuring the percentage of intact compound remaining as a function of time (Fig 3A, B and D)

Acta 1857 , 11271138 (2016)

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This step prevents contamination from the rubber stoppers, which may have collected dust or bacteria during storage and shipping