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10.1158/1535-7163.885.3.7 193 ZhangY.WeiZ.LiuW.WangJ.HeX.HuangH.et al (2017)

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cAMP accumulation assay Wild-type GLP-1R and GLP-1R mutants were cloned into the expression receptor pcDNA3.1/V5-His-TOPO vector (Invitrogen) at the HindIII and EcoRI sites by using the QuickChange site-directed mutagenesis and a flag-tag was inserted after native signal sequence (see primers in Supplementary Table 2)