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Probing position N108, we found that incorporation of any other amino acid resulted in a substantially reduced formation of cyanidin ( 8 ) suggesting that the hydrogen-bond interaction of the asparagine to the flavonoid substrate is critical for dehydratase activity, while GSH-transferase activity was comparatively less affected for N108 variants comprising an alanine, histidine, tyrosine or tryptophan (Fig
Batch culture of the engineered strain W303-1b/FGP for GSH production The batch fermentation was operated in a 10-L fermentor (China Beauty)
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