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The optical density (OD) values for the standards of all immunoassays tested were within the range established by the manufacturers, except for the last point of the standard curve (the most concentrated), which was higher than the declared in the human free BDNF Quantikine ELISA (#DBD00, R&D Systems), the high sensitivity pro-BDNF human ELISA kit (#SK00752-09, Aviscera Bioscience), the human pro-BDNF ELISA (#EH4255, FineTest) and the human BDNF ELISA kit (#EH0043, FineTest) (Supplementary Figures S2, S3 and S4)