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The preservative may still be working

The individual studies, published in Cephalgia in 2010, and the pooled results, published in Headache in 2010, both showed a significant decrease in the number of headache days (per 28 days) with eight to nine fewer headache days at 24 weeks as well as a significant improvement in quality of life in the Botox group

[PMID: 10198228] PubMed record related to this compound for laboratory literature review

The clinical dosage range spans 2.510 mg per subcutaneous injection, titrated over 4 weeks before reaching a maintenance dose of 510 mg, five times weekly

The ideal approach depends on individual circumstances