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Combining real-world datasets in diverse populations is essential to achieve broader applicability
If you just think about it, you wake up in the morning and this is what you are going to eat every day
& Rohmer, V
The protein was then incubated with a buffer consisting of 20 mM HEPES, pH 7.4, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 50 M tirzepatide, 20 g/mL Nb35, 0.03% (w/v) LMNG, 0.01% (w/v) GDN, 0.008% (w/v) CHS and 30 g/mL His-tagged TEV protease on the column overnight at 4 C
Liu J, Atamna H, Kuratsune H, Ames BN (2002)
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