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Short-lived enzymesubstrate interactions without stable complex formation are also in accordance with infinite k cat values and an inefficient competitive inhibition of ScGrx7

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The free energy of a typical reaction is calculated directly from its E o by the Nernst equation as shown below, where n is the number of electrons involved in the reaction and F is the Faraday constant (23.06 kcal/volt/mol or 94.4 kJ/volt/mol): G = nF E o For the oxidation of NADH, the standard biological reduction potential is 52.6 kcal/mole