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If youve been feeling: Worn out no matter how much you sleep Mentally slow or forgetful Weak, pale, or dizzy Numbness in hands or feet Low mood or anxious for no reason these could all be signs of a vitamin B12 deficiency

Subsequently, the HUVECs are stained with a cell-permeable dye calcein AM (Corning, catalog number 354216) for 30 min, and then observed for capillary-like tubule formation using an inverted microscope

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Biochem Biophys Res Commun (2001) 282:7358