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After a final wash step, a tetramethylbenzidine (TMB) peroxidase substrate solution (KPL Inc.) was added to each well and absorbance of the developed color was measured by a colorimetric plate reader (Molecular Device)

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Leptin receptor signaling in POMC neurons is required for normal body weight homeostasis

The PNMT encoded enzyme is also involved in the synthesis of several of the trace amine neurotransmitters