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The freeze-thaw cycle can be harsh on peptide structures and may cause some of the peptide to fall out of the solution or degrade
But is there more you can do in that interim instead of carrying that small (or large) annoying anxiety in the back of your mind until that retest date rolls around in next years calendar
Autophagy 3 , 374376 (2007)
Applications Enzyme-linked immunosorbent assay for Antigen Detection
Schooneman et al., 2013)
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