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Collagen is the most abundant protein in the body, integral to the structure of skin, hair, nails, and connective tissues

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7 L of RNA was added to the RT and buffer mixture prepared with random hexamers and oligoDT primers as per kit instructions, and rtPCR carried out with the following programme: 10 min at 25C, 30 min at 55C and 5 min at 85C

Some research protocols account for this by using comparatively lower dosing ranges in female study cohorts, reflecting a generally increased sensitivity to IGF-1 receptor activation a pattern also documented in broader growth hormone axis research, not unique to IGF-1 LR3 specifically