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Circular dichroism spectroscopy, which we did to understand the secondary structure of our best candidate bovine IF, which indicated a predominantly helical secondary structure consistent with native folding, while computational docking predicted bovine and human IF to have optimal geometric complementarity and stabilising energies with B12, in agreement with our experimental binding data

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Though those are nearly always elevated in cases of B12 deficiency, there are rare cases of severe B12 deficiency manifesting with normal B12 levels in the blood, normal MMA levels, and normal homocysteine