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Design and synthesis of glutathione analogues
The peptide effectively counters neuronal damage, including axonal and neuronal necrosis, which are critical factors in functional recovery
This discovery represented a major advance in understanding enzymatic antioxidant defence mechanisms and highlighted the biological importance of selenium, which was later recognised as an essential trace element due to its role in selenoproteins such as GPx
For experiments where extracellular arginine was depleted, 300 ng ml 1 recARG1 (Bio Cancer, PEG-BCT-100, rhArgIpeg5000) or RPMI medium without lysine and arginine (Thermo Scientific, 88365, substituted with 0.21857923 mM L-lysine hydrochloride and for control medium also with 1.1494253 mM arginine) was used and was present throughout the entire stimulation