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The nuclei were pelleted by centrifuging at 550 g for 10 min, at 4 C, and washed once in Isotonic Buffer, incubated for 10 min on ice, centrifuged again, and resuspended in Diffusion Buffer (20 mM HEPES pH 7.5, 110 mM KOAc, 5 mM NaCl, 2 mM MgCl 2 , 0.25 M sucrose) to a final concentration of 5000 nuclei/l