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By "smoothness" I mean the correct definition of the word, which is a fluid consistent action free of felt or heard mechanical noise or points of roughness, not to be confused with "free-spinning" which is the light operation without much resistance, often erroneously called "smoothness"
youll rely heavily on the movement of the tip, or your finger on the line, to determine lighter bites
Gene validation To validate the candidate genes from screening, sgRNAs from the parent library were cloned into pLentiCRISPRv2 (Addgene plasmid 52961)
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