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Unincorporated nucleotides
allow to dry
The protocol can be performed manually or using an automated platform for high throughput processing
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive
Kanamycin is commonly used to select for cosmid vectors
Kanamycin Sulfate Solution Name:Kanamycin Sulfate Solution Unincorporated nucleotidesKanamycin inhibits bacterial protein synthesis by binding 70S ribosomes resulting in dysfunctional translation of mRNA. Kanamycin is effective against both Gram ( ) and Gram (+) bacteria. Resistance to kanamycin is conferred by an aminoglycoside phosphotransferase that modifies the antibiotic, preventing its interaction with ribosomes. Kanamycin is commonly used to select for cosmid vectors.
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