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Secondly Im consuming 500 mcg once per day for thirty days, whereas other protocols vary the dose and frequency

Pyridoxine hydrochloride (Vitamin B6) plays an essential role in the nervous system, especially in the enzymatically controlled neurotransmitter metabolism

Diabetes Care 43 (3), 508511

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A 1-day shift in timing changes peak exposure by roughly 10 to 15%