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The pKa, of imidazole rings varies according to the microenvironment that protein folds in, and this huge pKa fluctuation enables the proteins histidine binding with metals (Miyagi and Nakazawa, 2024)
In this case control plates consisted of DMSO-supplemented medium
Science 333 , 11091112 (2011)
In dogs under physiologic strain, oxidative stress can be higher, which is where antioxidant support may be considered
avoid placing it in a freezer door where temperatures fluctuate wildly
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