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Vascular basement membrane thickening in diabetic retinopathy

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The DNA sequence spanning the CBS gene ARE motif located 4970 bp upstream of the transcript start site and its mutants were cloned into a GFP expressing reporter pEGFP-N1 (PT3054, Clontech) with sequence specific primers (Supplementary Table 1)

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Neutralizing Free Radicals Antioxidants like vitamin C , vitamin E, glutathione , and flavonoids neutralize free radicals, reducing cellular damage and inhibiting the overproduction of melanin triggered by oxidative stress