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Mice were anesthetized via intraperitoneal injection of a ketamine-xylazine cocktail (75 mg/kg and 16 mg/kg, respectively) delivered in a single syringe

The mechanism is direct: less 5-MTHF less methionine regeneration less SAM less DNMT activity progressive CpG demethylation at aging-clock sites accelerated measured epigenetic age

This gives a more sustained prompt for growth hormone production compared with a series of short spikes

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