The mice were intracardially perfused with phosphate-buffered saline (PBS) followed by cold 4% paraformaldehyde (PFA) in PBS
This may require more frequent injections to get the weekly dose you need
Avoid lying down immediately after eating, and stay away from greasy, fried, or very spicy foods as they can worsen symptoms
Neeland, Linge, and Birkenfeld (2024), writing in Diabetes, Obesity and Metabolism , conclude that skeletal muscle changes with GLP-1 receptor agonist treatment appear adaptive: reductions in muscle volume are commensurate with what is expected given aging, disease status, and weight loss achieved, and improvements in insulin sensitivity and muscle fat infiltration likely contribute to improved muscle quality
When the non-fluorescent DCFH is oxidized by ROS, it will be converted to highly fluorescent DCF