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A landmark randomized, cross-over clinical trial published in the International Journal of Clinical Nutrition & Dietetics by [Fanelli et al

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doi: 10.1016/j.nano.2009.07.002 179 TheuretzbacherU.OuttersonK.EngelA.KarlnA

We hypothesize that (i) the slow inhibition observed in the biochemical assay with the recombinant protein with some of the compounds is due to their preferential binding to a slowly populated conformer of the NADP + -TGR(H) complex and (ii) this conformer is already populated in the worm cell interior accounting for the more rapid inhibition of TGR observed in ex vivo worms with the slow inhibitors