Microbiota metabolite butyrate differentially regulates Th1 and Th17 cells differentiation and function in induction of colitis
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Real-time-PCR analysis After the mice were sacrificed, total RNA was extracted from frozen tissues using Trizol reagent (Invitrogen), according to the manufacturers instructions
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PubMed Practice guidelines for preoperative fasting and the use of pharmacologic agents to reduce the risk of pulmonary aspiration: Application to healthy patients undergoing elective procedures: An updated report by the American Society of Anesthesiologists task torce on preoperative fasting and the use of pharmacologic agents to reduce the risk of pulmonary aspiration