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Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)
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(Source 11) On the other hand, the American diet tends to be high in low-quality dairy products that may contain refined sugar, fillers, preservatives, and other ingredients that burden the system and require detoxification
GSH is in a class of molecules called antioxidants, whose primary job is to alleviate oxidative stress
Therefore, it is hypothesized that an increased GSH/GSSG ratio is a measure of the antioxidant effect of topical glutathione
