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Step 2: Support the Precursors

Tunnicliffe HE (1925)

Thus, independent of whether the experiments were started with reduced or oxidized proteins, most of strep-MBP-ROXY9 was in the reduced state in the presence of GSH with spurious amounts of GSSG, while strep-MBP-GRXC2 became oxidized, with about half of the proteins in the sample being glutathionylated and half containing the intramolecular disulfide