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Compliance note Research compound

Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

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In vitro treatment with GSSSG and LPS Mller and BV-2 cells were maintained and expanded in 10-cm dishes containing DMEM (10% FBS) in a 5% CO 2 incubator at 37 C

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