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Characterization The morphological characteristics of the samples were acquired by using a FEI Tecnai 12 transmission electron microscope (TEM) (Philips, Eindhoven, Netherlands) operating at an acceleration voltage of 120 kV

Therefore, we should consider this factor and how to address cellular tolerance to ferroptosis before using ferroptosis-inducing agents to treat prostate cancer

It enhances both synthesis and breakdown of extracellular matrix components, such as collagen, glycosaminoglycans (including dermatan and chondroitin sulfate), and decorin, and regulates metalloproteinases and their inhibitors (Pickart, Vasquez-Soltero, and Margolina 2015)

To isolate fractionated adipocytes, fresh gWAT was minced and digested for 1 h in PBS containing 10 mM CaCl 2 , 2.4 U ml 1 Dispase II (Roche) and 1.5 U ml 1 collagenase D (Roche)