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Author: Emma Beukers Osteoporosis is a debilitating skeletal condition characterized by low bone density, causing bone fragility and increased risk of bone fractures [1]

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Muscle samples were homogenized and protein concentration was determined using the Bradford method, as described previously [30]

CR also reduced DNA hypermethylation at the E2F-1 (active transcription factor for p16INK4a) binding site in nurse cells, thereby preventing downregulation of p16INK4a (Tramontano et al., 2020)

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