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This was about giving my body what it needed to function optimally, to quiet the noise that had been drowning out my joy for decades
Insulin-secreting BRIN-BD11 cells were cultured in sterile tissue culture flasks (Corning, Glass Works, UK) using tissue culture medium RPMI-1640 containing 10% (v/v) foetal bovine serum, 1% (v/v) antibiotics (100 U/ml penicillin, 0.1 mg/ml streptomycin) and 11.1 mmol/l glucose [29]
In the presence of methylene blue there is a very considerable increase in the production of [14C]carbon dioxide from [14C-1]glucose, and a corresponding decrease in the label in lactate and pyruvate, although the total radioactivity in (lactate + pyruvate + carbon dioxide) is the same as in the control incubation
If you dont need the extra discharge capability, you could end up wasting money
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