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Systematically investigating the pharmacological mechanism of Dazhu Hongjingtian in the prevention and treatment of acute mountain sickness by integrating UPLC/Q-TOF-MS/MS analysis and network pharmacology

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developed CSPB NPs that activate the PKA/p-CREB/IL-33 axis to induce the expression of anti-inflammatory factors, thereby indirectly inhibiting NF-B signal activation through the ST2/TRAF6 pathway, achieving a composite regulation of positive signal activation-negative pathway inhibition. In an IRI mouse model, this system reduced p-p65 levels by 43% while upregulating IL-33 expression by 2.8-fold, indicating its ability to maintain inflammatory homeostasis through positive and negative regulation of signaling pathways [83]