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Variations in these genes can reduce the efficiency of detoxification processes, increasing the risk of toxicity and oxidative damage

This can be prevented by derivatizing GSH with the alkylating agent N -ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by back-extraction of excess NEM from the deproteinized samples with dichloromethane

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However, if trace amounts (below 0.5%) are used for preservation and are non-intoxicating, some scholars consider it permissible

Biao Li, Shanghai Jiao Tong University, China Updates Copyright 2020 Wang, Yang, Ding, Li, You, Dai and Zhao