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The Activity Index (AI) was calculated according to the formula: Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and MIC/MBC ratio of PVA/CH-SeNPs (1%) nano-composite film The MIC and MBC values were determined for the PVA/CH-SeNPs nano-composite film (1%), which exhibited the most pronounced antimicrobial activity in preliminary diffusion assays using a two-fold serial dilution method, following Clinical and Laboratory Standards Institute (CLSI) guidelines [18]

[PubMed] [PubMed] Krishnaswamy, K., & Lakshmi, A

This becomes more relevant with extended use, typically after several months

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