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No invasive techniques are used, and no anesthesia is required

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The stratum corneum, an integral part of the skin barrier, is formed through the differentiation of keratinocytes and serves as the primary protective layer against external injury and stimulation (206)

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Its discovery opened a new chapter in sleep science, establishing that the brain produces discrete peptide signals capable of modulating sleep architecture a concept that has since expanded into a broader field of sleep-regulatory neuropeptide research