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French JB et al

While B12 is generally safe, caution is needed in specific clinical situations: Lebers Disease (Hereditary Optic Neuropathy): B12 injections are generally contraindicated or should be used with extreme caution, as they may potentially aggravate optic atrophy in this rare condition

Biotin supplements will likely only help hair grow back in people with a true deficiency in the vitamin

b) the widespread use of antibiotics in animal farming, which impairs B12 production by the animal gut microbiota

While there are no universally agreed-upon "optimal" ranges, practitioners and specialty lab testing companies may base these on the levels of micronutrients in the blood that are associated with the best health outcomes (not just the absence of disease) in clinical research studies