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Based on the aforementioned literature on ASD pathophysiology, we hypothesized that adults with ASD would show evidence of a dysregulated Gln-Glu cycle and heightened E/I balance, manifested by an increase in Gln levels, accompanied by potential alterations in Glu and lower GABA levels

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47 however, in the autism cerebellum, the observed decrease in GSH concentration relative to controls indicates a chronic deficit of reducing equivalents in this region

The concentration of anti-inflammatory and inflammatory cytokines was detected using LEGENDplex TM Rat Inflammatory Panel (Biolegend) or LEGENDplex TM Customer Panel (Biolegend) following the manufacturer's protocol and acquired via BD FACSAria III cell sorter (BD Biosciences, USA)

Subcutaneous Injection Sites Abdomen 2+ inches from navel, rotating around stomach area Outer Thighs Mid to upper outer thigh regions Upper Arms Back of upper arm (harder to self-inject) Upper Outer Quadrant of Buttocks Upper outer region for easy self-administration Proper Injection Technique Technique involves: Clean injection site with alcohol swab Pinch skin to create fold Insert needle at 45-90 angle into subcutaneous tissue Inject slowly and steadily Remove needle and apply gentle pressure without rubbing Critically: Rotate injection sites to never use the same spot consecutively Important Injection Considerations GHK-Cu specifically can cause injection site reactions ranging from mild to moderate pain due to copper peptide irritation, with possible redness, swelling, or knot formation lasting several days