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The diluted genomic DNA was added to a 10 l reaction mix containing 0.2 mg/ml BSA, 4 mM DTT, 0.10% Tween, 0.1 mM dTTP, 1X phi29 buffer, and 15 U phi 29 DNA polymerase

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A patient can be taking the same dose of thyroid medication and still see a change in TSH because body weight, digestion, and metabolic status are changing in the background