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In a study adopting 7,12-dimethyl-benz(a)anthracene- (DMBA-) induced breast cancer animal model, the combined use of GbE and TAM was demonstrated to a significant reduction in the tumor area, increase in the tumor necrosis area, and reduction in the number of proliferating cell nuclear antigen- (PCNA-) positive cells ( in vivo can enhance the inhibitory effects of TAM on the growth of subcutaneous tumors of MCF-7 breast cancer cells and synergistically induce autophagy and apoptosis in ER+ breast cancer cells via suppression of the AKT/mTOR pathway (Qi et al., 2016)