One individual per group was euthanized before addressing the next animal in each group to render differences arising from the time of day at euthanization (e.g., cecal mass changes resulting from differences in time post-food consumption) comparable across all groups
With a vitamin B12 deficiency comes the risk of suffering from megaloblastic anemia, including symptoms of fatigue, weakness, pale skin, lack of appetite, infertility, and unexplained weight loss
Any buildup is just going to prevent the peptide from penetrating into the areas it's actually needed
The Mayo Clinic notes that even at therapeutic mega-doses, side effects are uncommon and mild: headache, nausea, diarrhea, fatigue, and a tingling sensation in extremities
Patients with kidney disease, Leber's disease, megaloblastic anemia, and breastfeeding women should discuss the benefits and risks and dosage with their doctor