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The specific primers were designed to target the regions flanking the GR gene insertion site, ensuring the accurate identification of successful knockouts
However, given that the ratio of GSH to GSSG in the cytosol is approximately 100:1, it seems unlikely that cytosolic GSH is limiting in this way
Reactive oxygen and nitrogen species (Ros/rns) scavenging assays, oxidative stress biomarkers, and chromatographic/chemometric assays
a , Relative metabolite abundance of GSSG and GSH in whole cell lysate (WC) and isolated ER fractions from HEK293T cells treated with or without 1 mM BSO for 48 h
Two key enzymes, acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3), are involved in the biosynthesis and remodeling of PEs, activation of PUFAs, and insertion of PUFAs into membrane PLs (Doll et al., 2017)
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